Simoa單分子免疫陣列超靈敏細胞因子
檢測服務(wù)
產(chǎn)品介紹：
SimoaTM（Single Molecular Array）又稱作數(shù)字式ELISA，是基于Simoa HD-1數(shù)字式單分子免疫陣列分析儀，可直接對血清和血漿中蛋白、核酸等生物標志物進行檢測的技術(shù)。它的檢測靈敏度比傳統(tǒng)的免疫檢測技術(shù)平均高1000倍以上，將蛋白質(zhì)檢測技術(shù)直接帶入單分子、數(shù)字化檢測時代，成為fg級超低豐度蛋白質(zhì)檢測領(lǐng)域的優(yōu)勢技術(shù)。
技術(shù)原理：
1、將捕獲抗體偶聯(lián)到磁珠上，磁珠與目標抗原結(jié)合，目的抗原與檢測抗體結(jié)合并帶有梅標記。
2、洗滌磁珠以去除非特異性結(jié)合的蛋白，加入酶反應(yīng)底物。
3、將加入底物的磁珠轉(zhuǎn)入Simoa光盤上，將熒光信號封閉在小孔內(nèi)。
應(yīng)用領(lǐng)域：
Simoa技術(shù)可用于腫瘤、神經(jīng)、感染性疾病、免疫炎癥、miRNA檢測，特別是癌癥的早期檢測、術(shù)后的監(jiān)測和精準用藥，將極大推動精準醫(yī)療的發(fā)展。運用Simoa可檢測到的低濃度生物標志物進行準確分析，為生命科學(xué)研究、體外診斷、伴隨診斷和血液篩查等領(lǐng)域。目前在全球引用該技術(shù)發(fā)表的文獻有200多篇，包括高分雜志Nature, Nature Biotechnology, JEM, JAMA Neurology等。
參考文獻
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